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. 2014 May 1;25(9):1532–1542. doi: 10.1091/mbc.E13-10-0600

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© 2014 Miyamoto et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Cytohesin-2 is required for myelin formation. (A) Primary OPCs were cocultured with DRG neurons with or without 10 μM SecinH3 for 3 wk and stained with an anti-MBP antibody (red) and DAPI (blue). Representative images are shown. Scale bar: 50 μm. (B) The percentages of the MBP-positive myelin segments were determined (n = 7 fields in three experiments). (C) Cocultures were lysed and immunoblotted with an antibody against MBP or β-actin. Data were evaluated with the Student's t test (*, p < 0.01). (D) Schematic diagram describing the proposed regulatory mechanism of differentiation.