FIG. 2.
The peptide containing tyrosine 813 is autophosphorylated on tyrosine. 32P-labeled peptides in 2-D peptide maps corresponding to the lower of the two spots associated with tyrosine 813 of JAK2 (lane 1), and the upper (lane 2) and lower (lane3) spot associated with tyrosine 813 in JAK2(797-1129) were scraped from the cellulose plates and subjected to phosphoamino acid analysis. The origin and the migration of phosphotyrosine (ptyr), phosphothreonine (pthr), and phosphoserine (pser) standards are indicated. Spots seen below ptyr are due to incomplete acid hydrolysis of the peptide.