FIG. 4.
p73−/− but not p53−/− thymocytes were resistant to TNF-α-induced apoptosis. (A) Thymocytes were isolated from 4- to 6-week-old p73+/+ or p73−/− mice and treated with mouse TNF-α (50 ng/ml) for 8 h in the absence or presence of CHX. Cell death was determined by FACS analysis of the sub-G1 population. (B) p73+/+ or p73−/− thymocytes described for panel A were treated with the indicated doses of mouse TNF-α (50 ng/ml) for 5 h. Cell death was determined by annexin V binding and propidium iodide uptake. (C) p73+/+ or p73−/− thymocytes described for panel A were treated with dexamethasone (100 ng/ml) for 10 h. Cell death was determined by sub-G1 DNA content. (D) p53+/+ or p53−/− thymocytes were treated with (+) or without (−) human TNF-α (50 ng/ml) for 8 h.