FIG. 6.
Expression of hemidesmosomal plaques in COS-7 transfectants. Examples of COS-7 transfectants determined to be positive (A, B, C) or negative (D, E, and F) for expression of hemidesmosomal plaques. Panels A to C show COS-7 cells transfected with wild-type β4 + α6 integrin subunits. Panels C to E show COS-7 cells transfected with β4S1356D S1360D S1364D + α6 integrin subunits. The cells were grown overnight on coverslips and processed for indirect immunofluorescence. The cells were stained for HD1/plectin (B and E [red in panel C or F]) and α6β4 (A and D [green in panel C or F]) and analyzed for colocalization (yellow in panel C or F). Notice the hemidesmosomal plaques in panel C and only a thread-like pattern in panel F. Bar, 10 μm.