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. 2004 May;24(10):4351–4360. doi: 10.1128/MCB.24.10.4351-4360.2004

FIG. 7.

FIG. 7.

Quantitation of the effect of serine mutations on hemidesmosome dynamics. (A and B) COS-7 cells transfected with the indicated Ser→Ala (A) or Ser→Asp (B) phosphorylation mutants as described in Materials and Methods. (A) The Ser→Ala mutants were incubated in the absence or presence of EGF (100 ng/ml) overnight and processed for indirect immunofluorescence as described in the legend to Fig. 6 and Materials and Methods. The graph describes the percentage of positive cells showing the presence of hemidesmosomal plaques in relation to the wild type β4 (wt; 100%). (B) The Ser→Asp mutants were incubated overnight on coverslips and then processed for indirect immunofluorescence and analyzed as described for panel A. *, P < 0.05 versus wild type; **, P < 0.05 versus wild type + EGF.