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. 2014 Aug 28;24(3):286–289. doi: 10.1097/CMR.0000000000000069

Fig. 2.

Fig. 2

(a) Macroscopy of the extracted orbital tumor: highly pigmented 6×2 cm large tumor, which ruptured during the extirpation. (b) Hematoxylin and eosin staining of the orbital tumor (×20): melanin-containing cells (melanocytes and melanophages) are abundant in the tumor. (c) Hematoxylin and eosin staining of the orbital tumor after using a bleaching method for melanins (×20): diffuse proliferation of the epithelioid cells and spindle cells, and low-grade nuclear atypia were seen. Small nucleoli were seen in some areas of the tissue. (d) HMB (Human Melanoma Black)-45 staining (×20): positive for atypical cells. (e) Melan-A staining (×20): positive for atypical cells. (f) Hematoxylin and eosin staining of the orbital tumor after using a bleaching method for melanins (×20): vast areas of necrosis were seen.