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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Eur J Immunol. 2014 Feb 20;44(5):1467–1479. doi: 10.1002/eji.201344063

Figure 1. FSTL-1 is involved in the modulation of the inflammatory response in vivo.

Figure 1

(A) DBA/1 mice were injected i.p. with 50 µg of LPS and serum was analyzed by FSTL-1 ELISA. The results are expressed as the mean+SEM, n=4 mice/group from one experiment representative of three performed.*p < 0.05 versus PBS, Mann-Whitney test. (B) DBA/1 mice were injected in the hind footpads with 2.5 µg of LPS and euthanized on day 3 after treatment. RNA from paw tissue was assayed by QRT-PCR for FSTL-1. The graph represents a fold change in mRNA level compared with control. The results are expressed as the mean+SEM, n=4 mice/group from one experiment representative of three performed.*p < 0.01 versus control, Mann-Whitney test. (C) Serum samples from healthy volunteers and patients with sepsis were assayed for FSTL-1 by ELISA. Each circle represents an individual subject. Statistical significance determined by Mann-Whitney test. (D) Schematic representation of the gene knockout strategy. (E) Wild type (WT) and FSTL-1 null (KO) mice were injected i.p. with 200 µg of LPS for 3 h and sera were analyzed by ELISA. Each circle represents an individual mouse. The data shown are from one representative experiment of two performed. *IL-1β level was below the 150 pg/mL detection limit. IL-1β in the sera of unstimulated mice was below the level of detection (data not shown). Statistical significance determined by Mann-Whitney test.