A. An example of CD146 staining on CD8+ T cells from a representative healthy donor. Gating was performed by first forward versus side light scatter to identify lymphocytes, then CD8+ T cells were gated using a dot plot showing CD3 versus CD8.
B. The percentage of CD8+ T cells expressing CD146 in the peripheral blood of healthy donors as a function of age. There were no significant changes in the percentage of CD146+CD8+ T cells in either males or females with increasing age.
C. The percentage of CD8+ T cells expressing CD146 in the peripheral blood of healthy donors by gender. Bars indicate medians and interquartile ranges. Using the Mann Whitney test, no significant association with gender was observed.