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. Author manuscript; available in PMC: 2015 May 1.

Published in final edited form as: Clin Immunol. 2014 Feb 13;152(0):36–47. doi: 10.1016/j.clim.2014.01.009

Stimulation of sorted CD8+CD146+ T cells and CD8+CD146− T cells from patients with Behcet’s or birdshot uveitis for 5 days in vitro with CD3/CD28 without the addition of any exogenous polarizing cytokines.

A. Intracellular IFN-γ and IL-17A in CD8+CD146− T cells from a representative patient with Behcet’s disease.

B. Intracellular IFN-γ and IL-17A in CD8+CD146+ T cells from a representative patient with Behcet’s disease.

C. Composite results of these experiments examining intracellular IFN-γ and IL-17A from 7 patients (5 Sarcoidosis, 1 Behcet’s and 1birdshot). Sarcoidosis is indicated as hollow circle Behcet’s is indicated by the inverted dark triangle and the birdshot by the dark square. Significance was determined using the Wilcoxon matched pairs test and the bars indicate the mean SEM with 95% confidence intervals.

D. Sorted CD8+CD146− and CD8+CD146− cells stimulated for 5 days with CD3/CD28 both demonstrated an increase in CD146 expression, but IL17 production was primarily associated with CD146 expression in only the CD8+CD146+ sorted cells. Shown here are representative data from one healthy donor as well as one sarcoid patient and one Behcet’s disease patient.

Stimulation of sorted CD8+CD146+ T cells and CD8+CD146− T cells from patients with Behcet’s or birdshot uveitis for 5 days in vitro with CD3/CD28 without the addition of any exogenous polarizing cytokines.

A. Intracellular IFN-γ and IL-17A in CD8+CD146− T cells from a representative patient with Behcet’s disease.

B. Intracellular IFN-γ and IL-17A in CD8+CD146+ T cells from a representative patient with Behcet’s disease.

C. Composite results of these experiments examining intracellular IFN-γ and IL-17A from 7 patients (5 Sarcoidosis, 1 Behcet’s and 1birdshot). Sarcoidosis is indicated as hollow circle Behcet’s is indicated by the inverted dark triangle and the birdshot by the dark square. Significance was determined using the Wilcoxon matched pairs test and the bars indicate the mean SEM with 95% confidence intervals.

D. Sorted CD8+CD146− and CD8+CD146− cells stimulated for 5 days with CD3/CD28 both demonstrated an increase in CD146 expression, but IL17 production was primarily associated with CD146 expression in only the CD8+CD146+ sorted cells. Shown here are representative data from one healthy donor as well as one sarcoid patient and one Behcet’s disease patient.