Table I.

Oligonucleotides used for reverse transcription and amplification of bisulfite treated small RNA

Target	Expc	Oligonucleotides(s)d
tRNAAsp(GUC)	RT	5′CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTGGCTTCCCA3′
tRNAAsp(GUC)	PCR	5′TTTTTGGTAGTATAGTGG3′, 5′CTGGTGTCGTGGAGT3′
tRNAGly(GCC)	RT	5′CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTGGTAATACACA3′
tRNAGly(GCC)	PCR	5′GTAGTTGGTTTAGTGGTAGA3′, 5′CTGGTGTCGTGGAGT3′
tRNATyr(GUA), Ua	RT	5′CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTGGTCCTTCC3′
tRNATyr(GUA), Ua	PCR	5′AATTGGTAGAGTATGTGATTGTAGAGTATG3′, 5′CTGGTGTCGTGGAGT3′
tRNATyr(GUA), Sb	RT	5′CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTGGTCCTTCC3′
tRNATyr(GUA), Sb	PCR	5′GAGTATGTGATTGTAGATTATAGGGT3′, 5′CTGGTGTCGTGGAGT3′
tRNAVal(AAC)	RT	5′GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGGTAATACACCAAT3′
tRNAVal(AAC)	PCR	5′TGATGGTTTAGGTGGTTATGA3′, 5′GTGCAGGGTCCGAGGT3′

^aU, unspliced

^bS, spliced

^cRT, reverse transcription

^dstem loops in RT primers are underlined