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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Radiother Oncol. 2014 Jan 16;110(3):538–545. doi: 10.1016/j.radonc.2013.10.040

Figure 5. Rad51 and Exo1 silencing in A549 cells abrogates the IR expansion phenotype and induces CD133 IR sensitivity.

Figure 5

(A) Exo1 and Rad51 were silenced via lentiviral transduction in A549 cells. Mixed populations were irradiated with 4Gy IR and the percent CD133+ cells 72 hours post IR were measured.

(B) CD133+ cells were magnetically separated from CD133− cells and IR sensitivity was measured via clonogenic survival. Cells were treated with either 4Gy or 8Gy and permitted to grow for 10–14 days prior to survival analysis.