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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Eur J Immunol. 2014 Feb 27;44(5):1433–1443. doi: 10.1002/eji.201344266

Figure 6. PPS14-specific IgG responses to PPS14+[MSA]-beads are enhanced by TLR ligand adjuvants, but not alum.

Figure 6

BALB/c mice (n=7) were immunized at day 0 and 14, with 2×108 PPS14+[MSA]-beads containing 240ng of PPS14 attached to the beads. Beads were admixed for 1 hour at room temperature before immunization with 25µg CpG-ODN, 50µg Pam3CSK4 (Pam3Cys) or 20µg synthetic monophosphoryl lipid A (MPL). No alum was added. (A) serum titers of MSA- and PPS14-specific IgG and IgM. (B) Serum titers of IgG isotypes during the primary (day 14) and secondary (day 21) responses. For serum titers of anti-MSA IgG, only secondary responses are shown because primary responses were mostly undetectable. One of two independent experiments is shown. *p < 0.05 (every group relative to mice immunized with PPS14+[MSA]-beads admixed with CpG-ODN). #p < 0.05 (primary [day 14] versus secondary [day 21] responses); Student's t-test.