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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Biotechnol Bioeng. 2013 Dec 28;111(6):1200–1209. doi: 10.1002/bit.25170

Figure 1. Schematic of recombinant constructs used in this work.

Figure 1

(A) We created recombinant forms of both VSV-WT and the M51R mutant containing a point mutation in the matrix protein that abolishes the ability to inhibit host innate immune response, using a reverse genetics system. We further altered these strains by inserting DsRed-Express or other fluorescent protein in the 5th position of the genome. (B) We also created an IFIT2 reporter construct by inserting the IFIT2 promoter containing the ISRE site into a ZsGreen1 expression plasmid. We sub-cloned this construct into a lentiviral vector and transduced onto PC3 cells to stably integrate this reporter transgene.