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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Biotechnol Bioeng. 2013 Dec 28;111(6):1200–1209. doi: 10.1002/bit.25170

Figure 2. Insertion of fluorescent protein into VSV genome does not attenuate replication.

Figure 2

PC-3 IFIT2-ZsGreen1 reporter cells were infected at an MOI of 50 with either the VSV-WT-DsRed-Express (filled triangles), VSV-M51R-DsRed-Express (filled circles), or the non-fluorescent parent of each strain (open triangles, open circles, respectively). Supernatants were harvested at 4, 8, 12, 24 HPI and titered for infectious virus by plaque assay. Each data point represents the mean of 3 biological replicates with the error bars representing 1 standard deviation. We found that the fluorescent strains replicated on the target reporter cells similarly to their non-fluorescent parent strains.