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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Biotechnol Bioeng. 2013 Dec 28;111(6):1200–1209. doi: 10.1002/bit.25170

Figure 5. Flow cytometry results confirm both viral and IFIT2 reporter function.

Figure 5

PC-3 IFIT2-ZsGreen1 reporter cells were infected with VSV-WT-DsRed-Ex or VSV-M51R-DsRed-Ex at MOI 50. 20 HPI cells were harvested and immunostained for VSV-G protein on the cell surface, and analyzed by flow cytometry. In both strains DsRed expression was found to be correlated with VSV-G protein although in the M51R infection, a small population of cells (~5%) were G positive but not DsRed positive, suggesting it is a somewhat less sensitive indicator of infection. The results also demonstrate that while the WT infection suppresses the activation of IFIT2, the M51R strongly induces its activity.