PC-3 IFIT2-ZsGreen1 reporter cells were infected with VSV-WT-DsRed-Ex or VSV-M51R-DsRed-Ex at MOI 50. (A) At 4, 12, 24 HPI total cell lysates were harvested and Western blotted for Mx1 protein expression. Two biological replicates were performed at each timepoint (representative blot shown).
(B). Band intensities were determined using Image Studio(LiCor) software. Each Mx1 band was normalized to β-actin band for that sample, replicates were averaged and expressed as fold over mock infected cells (harvested at 12 hrs). Results demonstrate that M51R strongly induced Mx1 expression while WT largely suppressed its activity.