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. 2004 May;24(10):4151–4165. doi: 10.1128/MCB.24.10.4151-4165.2004

FIG. 3.

FIG. 3.

DNA damage-induced Sae2 phosphorylation in checkpoint mutants. Strains expressing Sae2-HA3 from the SAE2 promoter in a bar1Δ background were as follows: wild type (wt) (DMP3909/7D), tel1Δ (DMP3920/16D), mec1Δ sml1Δ (DMP3919/3B), mec1Δ tel1Δ sml1Δ (DMP3919/11A), rad53Δ mec1Δ sml1Δ (DMP4295/10A), chk1Δ mec1Δ sml1Δ (DMP4297/5A), chk1Δ tel1Δ (DMP4296/1D), tel1Δ rad53Δ sml1Δ (DMP4298/2B), rad50Δ (DMP4048/7D), rad50Δ tel1Δ (DMP4050/17A), rad50Δ mec1Δ sml1Δ (DMP4052/18A), chk1Δ (DMP4141/12C), rad53Δ sml1Δ (DMP4106/27A), mrc1Δ (DMP4105/3B), rad9Δ (DMP4049/5C), ddc1Δ (YLL1345.6), ddc1Δ rad9Δ (YLL1346.2), mec3Δ (DMP4138/7C), rad17Δ (DMP4137/1D), and rad24Δ (DMP4137/17A). Cell cultures were arrested in G1 with α-factor and then transferred in YEPD containing 20 mU of bleomycin/ml and 1 μg of α-factor/ml (+bleo + α factor). Time zero (αf) corresponds to cell samples taken immediately before bleomycin addition. Protein extracts prepared from cell samples collected at the indicated times were subjected to Western blot analysis with anti-HA antibodies. exp, exponentially growing cells.