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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Clin Immunol. 2014 Feb 14;152(0):25–35. doi: 10.1016/j.clim.2014.02.002

Figure 5.

Figure 5

Cigarette smoke (CS) suppresses lung APC-induced splenic T cell proliferation but enhances systemic APC function in DQ8 mice. A) Splenic CD4+ T cells from CII-primed mice exposed to CS or control “non-smokers” (NS) were cultured with dendritic cells (DCs) and B cells isolated from lungs (L) and spleen (S) and challenged with CII (20 µg/ml). T cell response was suppressed when DCs and B cells from lungs were used as APCs in CS mice. In NS mice, DCs from lungs (L DC) led to a significantly higher T cell response than splenic DCs (S DC), while B cells did not show any significant difference. B) Splenic DCs from CS mice were cultured with CD4 cells from CS or NS mice. Similarly, DCs from NS mice were cultured with CD4 T cells from CS and NS mice. C) DCs and B cells isolated from lungs and spleen of CS mice were tested as APCs for native and Citrullinated type II collagen (CIT-CII) in DQ8 mice. D) DCs from lungs and spleen were cultured with splenic CD4 T cells in DR4 mice exposed to CS. Enough B cells from lungs of DR4 mice could not be obtained. All experiments were done 2–3 times with cells pooled from 2 to 3 mice/each experiment.