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. Author manuscript; available in PMC: 2014 Apr 29.
Published in final edited form as: Int J Oncol. 2011 Jul 1;39(5):1205–1211. doi: 10.3892/ijo.2011.1111

Figure 1.

Figure 1

Identification of senescence-associated miRNAs (SA-miRs) in NHK. (A) Schematic strategy for the identification of SA-miRs in NHK. (B) Confirmation of the up-regulation of five SA-miRs (miR-34a, 137, 181a, 449a, and 668) during senescence of NHK. Three independent cultures of NHK derived from different donors were serially propagated and used in this assay. The levels of mature SA-miR expression were measured by quantitative real-time RT-PCR. For each sample, the expression of miRNA was normalized to U6 snRNA. The value for miRNA from exponential replicating NHK was set at 1, and the relative amounts of miRNA from senescent NHK were plotted as fold induction. The graph shows the mean of three experiments. Error bar represents the standard deviation. (C) Regulation of SA-miRs in IR-induced premature senescence. Exponential replicating NHK were exposed to 0 or 5 Gy and harvested for determining the levels of the SA-miRs. The value for miRNA from control NHK (0 Gy) was set at 1, and the relative amounts of miRNA from NHK exposed IR were calculated. *Significantly different (p<0.0, Student’s t-test) from the control expression.