Figure 7.
SRC1 is recruited efficiently by both T3 and T4 to TRα1 in CHO and CV-1 cells by a mammalian 2-hybrid reaction. An expression vector encoding a Gal4 DNA binding domain (GBD)–SRC1 (LXXLL domains) fusion and an expression vector encoding a Gal4 activation domain–TRα1 fusion was transfected into CHO or CV-1 cells together with a GAL-17-mer luciferase reporter as described previously. The graphs represent means of 4 replicates. Error bars indicate SEMs. Vertical dashed and solid lines represent EC50s T3 and T4, respectively.