TABLE 2.
Absence of competition for chromatin binding between HMG familiesa
| Exptc | t80
|
R20sb
|
Effect on mobility | ||
|---|---|---|---|---|---|
| Avg time (s) ± SD | P value | Avg % ± SD | P value | ||
| HMGN1 | 8.81 ± 4.2 | <0.005 | 90.2 ± 7.0 | 0.12 | Increase |
| HMGN1 + HMGN2 | 4.17 ± 1.4 | 94.2 ± 3.76 | |||
| HMGN2 | 5.87 ± 1.9 | <0.02 | 88.6 ± 5.1 | 0.10 | Increase |
| HMGN2 + HMGN1 | 3.77 ± 0.9 | 93.1 ± 3.8 | |||
| HMGN1 | 5.40 ± 1.6 | >0.6 | 91.0 ± 2.0 | >0.5 | None |
| HMGN1 + HMGB1 | 4.68 ± 0.95 | 91.6 ± 4.0 | |||
| HMGN2 | 5.04 ± 0.8 | >0.9 | 94.5 ± 2.9 | >0.5 | None |
| HMGN2 + HMGA1 | 5.08 ± 1.0 | 93.7 ± 3.5 | |||
| HMGB1b | 5.20 ± 2.8 | <0.01 | 98.5 ± 2.0b | >0.4 | Decrease |
| HMGB1 + HMGA1 | 10.20 ± 2.3 | 97.3 ± 2.4 | |||
| HMGB1b | 5.78 ± 2.0 | >0.7 | 98.7 ± 3.0b | >0.8 | None |
| HMGB1 + HMGN2 | 5.39 ± 2.6 | 98.4 ± 0.9 | |||
a
Analysis was done as for Table 1.
b
For HMGB1, the R was measured at 45 s rather than at 20 s (i.e., R45s rather than R20s).
c
Mixed chromatin domains were tested in each experiment.