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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Mol Microbiol. 2014 Feb 20;92(1):1–9. doi: 10.1111/mmi.12534

Figure 2. Most divisome proteins remain at the division septum after cytoplasmic compartmentalization.

Figure 2

(A) Live cells expressing GFP-ZapA, ZipA-GFP, FtsA-GFP, GFP-FtsQ, GFP-FtsL or GFP-FtsI together with mCherryCYTO were studied at different stages of division by monitoring the localization pattern of GFP. Only images for cells expressing ZipA-GFP and mCherryCYTO are shown. (B) FRAP measurements were carried out to determine if the cytoplasm was still connected across the division septum (open). Summation of FRAP data from at least 150 cells of each strain at different stages of division. GFP-ZapA behaved as FtsZ-GFP, disassembling from the divisome before the cytoplasm was compartmentalized. For all other strains the cytoplasm compartmentalised whilst the GFP fusion was present at the septum. Since compartmentalization occurred during Stage 2, we generally did not perform FRAP measurements on cells during stage 3 (N.D.= Not Determined). Cells in which the GFP localization was relocated in the daughters were always closed (Stage 4). Note that FtsZ-GFP was included as a control.