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. 2014 Apr 30;34(18):6164–6176. doi: 10.1523/JNEUROSCI.3458-13.2014

Figure 5.

Figure 5.

Increased cell packing in the CA1 stratum pyramidale of Has3−/− mice. A, NeuN immunostaining of the hippocampus of Has3−/− mice and WT littermates. Low-magnification views of hippocampus (left). Scale bar, 200 μm. CA1 stratum pyramidale (middle). Scale bar, 100 μm. High-magnification views of the CA1 (right). Scale bar, 50 μm. B–E, Morphometric analyses of the stratum pyramidale of Has3−/− mice and WT littermates. The thickness of the stratum pyramidale (B), the number of neurons contained within the layer (per 200 μm long segment; C), the density of neurons contained within the layer (per 100 μm2; D), and the size of soma (longest diameter) of pyramidal neurons (E) were determined in NeuN-stained sections; n = 24 per genotype, ***p < 0.001; n.s., not significant (Student's t test).