TABLE 1.
Marker expression of retrovirus-transduced retinal explant culturesa
Antibodies against: | Population (%) of reactive cells from virus-encoded:
|
|
---|---|---|
EGFP | RX-IRES-EGFPb | |
GS | 31.1 ± 9.2 | 18.9 ± 7.8 |
Cyclin D3 | 2.78 ± 5.6 | 13.9 ± 2.3 |
NF160 | Not detected | 12.2 ± 6.7 |
PKC | 17.2 ± 4.9 | Not detected |
Retrovirus encoding EGFP or Rx/rax-IRES-EGFP was used for transduction of retina explant cultures, and after 2 weeks, frozen transverse sections were analyzed for marker expression by immunostaining. Populations (%) of antibody-reactive cells among the EGFP-positive cells were determined by counting independent samples.
IRES, internal ribosome entry site.