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. 2014 May 1;127(9):2071–2082. doi: 10.1242/jcs.144923

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Rab20 is required for the IFN-γ-induced delay in phagosome maturation. (A) The kinetics of LTR acquisition by phagosomes in RAW264.7 macrophages that were untreated (left panel) or treated with 200 U/ml IFN-γ (right panel). The cells were incubated with LTR for 30 min and subsequently incubated with 3-µm IgG-coated beads and analyzed by using live-cell imaging. Two independent experiments were performed. (B) Data points from A at 5 and 10 min were pooled. (C) The kinetics of LTR acquisition by phagosomes in macrophages expressing EGFP (left panel) or EGFP–Rab20T19N (right panel) after IFN-γ treatment. (D) Data points from C at 5 and 10 min were pooled (E) The kinetics of LTG acquisition by phagosomes in macrophages expressing scrambled shRNA (left panel) or Rab20 shRNA (right panel). (F) Data points from E at 5 and 10 min were pooled. The data show the mean±s.d. from at least three independent experiments. n, number of phagosomes analyzed. **P≤0.01, ***P≤0.001 (Student's two-tailed unpaired t-test).