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. 2014 May 1;127(9):2071–2082. doi: 10.1242/jcs.144923

Fig. 7.

Fig. 7.

Rab20 mediates the recruitment of Rabex-5 into phagosomes in IFN-γ-activated macrophages. (A) Western blot analysis of Rab20, Rab5a and Rabex-5 levels in macrophages stably expressing Rab20 shRNAs (2640, 2643 and 2644). Actin was used as a loading control. Con, control. (B) Quantitative analysis of the expression level of Rab20 in macrophages stably expressing Rab20 shRNAs. The intensity was calculated relative to the actin loading control and normalized to the relative intensity in control samples. The data show mean±s.e.m. of three independent experiments. *P≤0.05 (Student's two-tailed t-test). (C) Western blot analysis of Rab20, Rab5a and Rabex-5 levels in isolated phagosomes from control and Rab20-knockdown (KD) macrophages with or without IFN-γ treatment. (D) Quantitative analysis of the association of Rabex-5 with phagosomes in control and Rab20-knockdown macrophages with or without IFN-γ treatment. The relative Rabex-5 intensity was compared with the actin loading control and normalized against the 15 min time-point.