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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Cancer Res. 2014 Feb 27;74(8):2340–2350. doi: 10.1158/0008-5472.CAN-13-2625

Figure 1.

Figure 1

NF1-null melanoma cell lines express high levels of activated RAS. A) BRAF and NRAS status of the melanoma cell line panel (n=191). B) Activated RAS protein (RAS-GTP) was quantitated in select melanoma cell lines via immunoprecipitation with the RAS-binding domain of RAF (RAF-1 RBD) followed by immunoblot using pan-RAS and isoform selective NRAS and KRAS antibodies. Expression of NF1, total RAS (pan-RAS) and actinin (as a loading control) were measured by immunoblot from whole cell lysate (WCL). Alt = alteration, mut = mutant, WCL = whole cell lysate.