FIG. 2.

EMSA for NF-κB binding in BET-1A cells in vitro. (A) The cells were stimulated by TNF-α, and the nuclear extracts were isolated for EMSA as described in Materials and Methods. NF-κB was activated by stimulation with TNF-α. The specificity of the binding was ascertained by the supershift of the band with the antibody to p50, as well as to p65, compared to control antisera (normal goat immunoglobulin G). The addition of excess cold AP-1 probe showed no effect on NF-κB binding. (B) The activation of NF-κB induced by TNF-α was inhibited by incubation with EM at 10⁻⁶ M.