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Effect of EM on TNF-α-mediated degradation of IκBα: BET-1A cells were stimulated with TNF-α. Cytoplasmic lysates were separated by polyacrylamide gel electrophoresis and then analyzed by blotting them with anti-IκBα and anti-phosphorylated IκBα antibodies. The addition of TNF-α resulted in the degradation of IκBα within 5 min. Incubation with EM had no effect on the degradation of IκBα.
