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. Author manuscript; available in PMC: 2014 Apr 30.
Published in final edited form as: Angiogenesis. 2009;12(3):297–301. doi: 10.1007/s10456-009-9155-3

Fig. 1.

Fig. 1

Lectin-stained retinal whole mounts showing the zone of vaso-obliteration (VO) and neovascularization (NV). After oxygen incubation a central VO zone develops (a) that is quantified by manually outlining the edges of the VO area (b). After returning the mouse pups to room air the hypoxic retina in the VO zone triggers formation of retinal NV (c). In the traditional hand-measurement protocols all neovascular tufts (arrowheads) and clusters (arrow) are manually marked to quantify retinal NV (d)