TABLE 1.
Comparison of the inhibitory effect caused by phenyl-phenalenones on parasite proliferation, FRD and SDH from the mitochondrial fraction, and purified FRDa
| Drug | LC50 (μg/ml)
|
EC50 (μg/ml)
|
|||
|---|---|---|---|---|---|
| Promastigotes | Amastigotes | Mitochondrial fraction
|
Purified FRD | ||
| SDH | FRD | ||||
| Anigorufone | 12.0 (10.8-13.0) | 13.3 (7.3-24.2) | 33.5 (17.1-65.7) | 53.1 (33.2-84.7) | 89.0 (57.5-138.0) |
| REF5 | 24.2 (21.0-27.8) | 23.9 (15.1-37.9) | >80 | 78.8 (65.0-95.3) | 115 (72.6-182.2) |
| REF20 | 10.3 (7.3-14.5) | 10.5 (7.4-14.8) | 59.6 (51.8-68.6) | 47.8 (41.0-55.6) | 77.2 (49.8-119.6) |
| EP5 | 38.4 (28.4-51.9) | 15.6 (13.3-18.2) | >80 | 61.8 (46.3-82.5) | 100.0 (77.6-128.8) |
| EP6 | 68.7 (60.8-77.7) | 17.2 (10.9-26.6) | >100 | 33.3 (29.9-41.9) | 53.3 (33.6-84.5) |
a
LC50 and EC50 values were estimated by the procedure of Litchfield and Wilcoxon. The 95% confidence interval values are given in parentheses. Assays were carried out as described in Materials and Methods.