TABLE 3.

Genetic analysis of MexAB-OprM- and MexXY-overproducing strains of P. aeruginosa

Strain	Nucleotide sequencing dataa				Mean gene expressionb
	mexZc	mexRd	PA3721e	Vicinity of PA3721	mexX	mexA	PA3720
PAO1					1.0	1.0	1.0
PT629 (nalB)	ND	4 bp deleted at posi- tion 363			0.6	3.6	1.71
ATM4 (nalB agrZ)	CAG→TAG (C307T)	GCA→CCA (A66P)			482	6.5	0.11
WL22	48	44, 126	71, 153	G−73→A, A+13→T	168	9.2	2.9
WL24			71, 209		22.0	5.1	0.2
1113	GCC→-CC (A35−), ATG→ AT-(M34−)	126	71, 209	T−2→C, C inserted (−25)	29.6	4.4	5.5
1217	144, GAG→-AG (E157−)		71		98.5	6.0	0.5
1237	48, CAG→TAG (Q10Z)	44, 126	71, 153, 209, TCA→GCA (S46A)	A+13→T	272	7.7	3.7
1250	GGT→G--(G46−), GCG→- CG (A47−)		500 bp deleted		312	17.9	147
1562	144		71		129	8.5	1.7
1727		7-bp insertion between 355 and 361	71, GAT→GAA (D76E)		300	34.8	9.9
1738			71, 209, CTG→CCG (L61P)		82.2	11.7	224
2085	48	44, 126	71, 153	T−2→C	204	8.7	5.8
2151	GTC→GCC (V44A)	44, 126	71, 153, 209	T−2→C, A+13→T	110	2.6	6.7
2172	GGT→AGT (G46S)		71, ATG→ACG (M151T)		95.4	6.1	194

^aNucleotide differences compared with the PAO1 genome (www.pseudomonas.com).

^bRelative to PAO1; mean values from two independent experiments.

^c48, substitution GTC→GCC (V₄₈→A); 144, GCG→GTG (V₁₄₄→A).

^d44, substitution AAG→ATG (K₄₄→M); 126, GTG→GAG (V₁₂₆→E).

^e71, substitution GGG→GAG (G₇₁→E); 153, GAG→CAG (E₁₅₃→Q); 209, AGC→CGC (S₂₀₉→R).