FIG. 1.

The standard and sandwich culture methods. (A) Diagram of the standard and sandwich culture methods. In the standard culture method, limbal epithelial cells were cultured directly on feeder cells. In the sandwich culture method, feeder cells attached to the bottom of the polyethylene terephthalate (PET) membrane, and the cultured cells were seeded onto the inner side of the membrane. (B) Loading of the 3T3 feeder cells on the inverted PET membrane. Color images available online at www.liebertpub.com/tec