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. 2013 Nov 5;20(5):393–400. doi: 10.1089/ten.tec.2013.0246

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 2. — Limbal stem/progenitor cells (LSCs) cultured in single-cell suspension. (A) Morphology of LSC colonies. (B) Cell population doubling (PD) of limbal epithelial cells. (C) Relative mRNA levels of putative stem cell markers and maturation markers as evaluated by quantitative real-time–polymerase chain reaction (qRT-PCR). The expression of markers from single-cell culture using the standard method served as a control and was designated a value of 1. Asterisks indicate p<0.05 in comparison with results for the standard method.