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. 2013 Nov 5;20(5):393–400. doi: 10.1089/ten.tec.2013.0246

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 3. — Comparison of LSCs derived from cell cluster standard and sandwich cultures. (A) Morphology of colonies. (B) Cell population doubling of limbal epithelial cells. (C) Relative mRNA levels of the putative stem cell markers and maturation marker as evaluated by qRT-PCR. The expression of markers by control single-cell standard culture served as a control and was designated a value of 1. Asterisks indicate p<0.05 in comparison with results for the cluster standard method or the control method. Ctl, control; CST, cluster standard culture method; CSW, cluster sandwich culture method.