FIG. 5.

Impact of MnTE-2-PyP⁵⁺ on the abundance of protein-bound glutathione and cysteine in control and GALT-null Drosophila early larvae (L2) reared in the presence of dietary galactose. Protein thiolation is a marker of oxidative stress in biological systems. Samples tested were from control (open bars) and GALT-null (shaded bars) second instar (L2) larvae harvested from foods containing 200 mM galactose (in addition to 555 mM glucose) that did versus did not contain 10 μM MnTE-2-PyP⁵⁺, as indicated. Values plotted represent average±standard error of the mean, n=6. (A) Levels of protein-bound cysteine, and (B) levels of protein-bound glutathione, are plotted. Significant differences are indicated.