Fig. 1. human sera titration in ELISA-soluble Schistosoma mansoni adult worm antigen preparation (SWAP) and ELISA-soluble S. mansoni egg antigen (SEA). Antigen: SWAP or SEA (1 µg/mL, 3 µg/mL, respectively). Sera: endemic area positive sera and non-endemic area negative sera, diluted (1:50-1:300) in phosphate buffer saline (PBS). Conjugate: peroxidase labelled anti-human IgG conjugated to peroxidase (IgG-HRP) diluted (1:40.000-1:100.000) in PBS-Tween. Substrate: tetramethylbenzidine substrate solution.
