FIG. 3.

Uptake of diamidines by S. cerevisiae expressing TbAT1. (A) Yeast cells of strain RH2884 transformed with pRS416-MET25 were incubated with 2.5 μM [³H]diminazene in the presence (□) or absence (▪) of 1 mM unlabeled diminazene. Uptake rates were calculated by linear regression and found to be not significantly different from zero (P = 0.12) or very significantly different from zero (P < 0.0001), respectively, in the presence or absence of excess permeant. (B) Accumulation of 2.5 μM [³H]pentamidine by RH2884/pRS416-MET25 in the presence (□) or absence (▪) of 1 mM unlabeled pentamidine.