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. 2014 Feb 21;42(8):5270–5279. doi: 10.1093/nar/gku157

Figure 1.

Figure 1.

BCBP contains an unconventional miR398 target site. (A) Schematic presentation (drawn to scale) of the BCBP gene (At5G20230). The location of the miR398 target site is shown (red box), exons are represented by gray boxes and UTRs by white boxes. The sequence (Arabidopsis accession Col-0) of the miR398 complementary site (in bold) in the BCBP mRNA is aligned with sequences of both miR398a and miR398b/c. Cleavage was experimentally validated by a modified version of the 5′-RACE PCR (20). The arrow indicates the 5′ terminus cleavage product for the number of clones mentioned. (B) Degradome data (24) corresponding to the signatures matching At5G20230 in both Col-0 WT plants and xrn4 mutants. The arrows indicate the signatures that correspond to the miR398 target site identified in (A). The gene model is presented below.