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. 2014 Mar 13;42(8):5289–5301. doi: 10.1093/nar/gku137

Figure 3.

Figure 3.

Processing of ASRs is independent of DROSHA. (a) Sequences of precursor of ASR transcripts are shown. That of ASR1 is ENST00000387449; ASR2, NR_004393.1; ASR2, NR_004392.1; ASR4, NG_032096.1. Underlined sequences indicate mature ASRs. (b) Structures of ASR1, 2, 3 and 4 were predicted with sequences indicated in Figure 3a by the mfold web server. Highlighted regions indicate mature ASRs sequences. (c and d) Control siRNA (Ctrl.) or siDROSHA were transfected into L591 cells by Neon™ transfection system. (c) Expressions of DROSHA was evaluated by real-time PCR 3 days after the transfection. Expression levels relative to Ctrl. were normalized with GAPDH and shown. (d) Cells were cultured for 6 days, and then total RNAs were extracted. Amounts of ASRs and mature miR-21 were determined by real-time PCR. Data were shown as relative expression to the Ctrl. normalized with RNU6. Error bars indicate SD. *P < 0.05.