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. 2014 Feb 24;42(8):5347–5360. doi: 10.1093/nar/gku140

Table 1.

Parameters derived from SAXS

Guinier
GNOM
Sample Conc.a (ng/µl) qRg Rg (Å) I(0) (a.u.) qb Rg (Å) I(0) Dmax (Å)
APS BioCAT
+A Group II Intron 100 0.98–1.3 73.5 ± 0.8 324.0 ± 3.0 0.006–0.37 74.9 326.2 250
50 0.47–1.3 75.3 ± 0.9 139.0 ± 1.0 0.006–0.37 72.7 133.0 225
40 0.45–1.3 73.0 ± 1.0 120.2 ± 1.0 0.006–0.31 72.4 116.0 225
Inf. dil. 0.57–1.2 73.3 ± 0.9 73.3 235
NSLS X9
+A Group II Intron (DII-tRNA) 100 1.0–1.4 87.3 ± 1.2 131.7 ± 2.1 0.010–0.25 95.1 125.0 284
CHESS F2c
+A Group II Intron
0% Sucrose 40 0.71–1.3 71.2 ± 4.4 0.53 ± 0.03 0.0097–0.15 70.7 0.52 235
16.5% Sucrose 60 0.89–1.7 86.6 ± 3.9 0.70 ± 0.04 0.0097–0.15 83.8 0.64 235
32.5% Sucrose 50 1.09–1.9 105.8 ± 8.3 0.52 ± 0.06 0.0097–0.10 92.9 0.42 235
48.8% Sucrose 100 1.19–2.1 115.2 ± 5.6 0.87 ± 0.07 -n.d.- -n.d.- -n.d.-
65% Sucrose 50 1.22–2.2 118.6 ± 11.8 0.41 ± 0.07 -n.d.- -n.d.- -n.d.-

aWhere q = 4πsinθ/λ, where 2θ is the scattering angle, in units of Å-1.

bAs determined by U.V. absorbance.

cI(0) in units of (cm-1)