Table 1.
Guinier |
GNOM |
|||||||
---|---|---|---|---|---|---|---|---|
Sample | Conc.a (ng/µl) | qRg | Rg (Å) | I(0) (a.u.) | qb | Rg (Å) | I(0) | Dmax (Å) |
APS BioCAT | ||||||||
+A Group II Intron | 100 | 0.98–1.3 | 73.5 ± 0.8 | 324.0 ± 3.0 | 0.006–0.37 | 74.9 | 326.2 | 250 |
50 | 0.47–1.3 | 75.3 ± 0.9 | 139.0 ± 1.0 | 0.006–0.37 | 72.7 | 133.0 | 225 | |
40 | 0.45–1.3 | 73.0 ± 1.0 | 120.2 ± 1.0 | 0.006–0.31 | 72.4 | 116.0 | 225 | |
Inf. dil. | 0.57–1.2 | 73.3 ± 0.9 | 73.3 | 235 | ||||
NSLS X9 | ||||||||
+A Group II Intron (DII-tRNA) | 100 | 1.0–1.4 | 87.3 ± 1.2 | 131.7 ± 2.1 | 0.010–0.25 | 95.1 | 125.0 | 284 |
CHESS F2c | ||||||||
+A Group II Intron | ||||||||
0% Sucrose | 40 | 0.71–1.3 | 71.2 ± 4.4 | 0.53 ± 0.03 | 0.0097–0.15 | 70.7 | 0.52 | 235 |
16.5% Sucrose | 60 | 0.89–1.7 | 86.6 ± 3.9 | 0.70 ± 0.04 | 0.0097–0.15 | 83.8 | 0.64 | 235 |
32.5% Sucrose | 50 | 1.09–1.9 | 105.8 ± 8.3 | 0.52 ± 0.06 | 0.0097–0.10 | 92.9 | 0.42 | 235 |
48.8% Sucrose | 100 | 1.19–2.1 | 115.2 ± 5.6 | 0.87 ± 0.07 | -n.d.- | -n.d.- | -n.d.- | |
65% Sucrose | 50 | 1.22–2.2 | 118.6 ± 11.8 | 0.41 ± 0.07 | -n.d.- | -n.d.- | -n.d.- |
aWhere q = 4πsinθ/λ, where 2θ is the scattering angle, in units of Å-1.
bAs determined by U.V. absorbance.
cI(0) in units of (cm-1)