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. 2014 Feb 21;42(8):4922–4933. doi: 10.1093/nar/gku150

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© The Author(s) 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Formation of nucleosome duplexes and altosomes are H3(T118ph)-dependent. (A) (Top, unmod − AP): mass spectrum of unmodified HO (H3 [M+2H]²⁺ m/z expected: 7637, observed: 7637), (Middle, T118ph − AP): H3(T118ph) HO before AP treatment (H3(T118ph) [M+2H]²⁺ m/z expected: 7677, observed 7676) and (Bottom, T118ph + AP) H3(T118ph) HO after AP treatment (H3(T118): ([M+2H]²⁺ m/z expected: 7637, observed: 7634). (B and C) EMSA of mp2-187 and mp2-247 DNA’s, respectively, reconstituted with unmodified HO without AP treatment (lane 1), H3(T118ph) HO without AP (lane 2), unmodified HO after AP (lane 3) and H3(T118ph) HO after AP (lane 4).