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. 2014 Feb 21;42(8):4922–4933. doi: 10.1093/nar/gku150

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© The Author(s) 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Nucleosome duplexes are assembled by yNap1. EMSA of (A) unmodified HO and (B) H3(T118ph) containing HO reconstituted on mp2-187 by salt dialysis (lane 1, ‘R’), unmodified HO mixed with DNA in the absence of yNap1 (lane 2, ‘0’) and unmodified HO assembled on DNA in the presence of increasing amounts of yNap1 as indicated for each lane. (C) Quantification of the fraction of yNap1 assembled nucleosomes (‘nuc’, squares), nucleosome duplexes (‘ND’, triangles) and remaining free DNA (‘DNA’, diamonds) as a function of [Nap1] for unmodified (black) and H3(T118ph) HO (gray) from the data in (A) and (B). (D) Fraction of centrally positioned versus depositioned nucleosomes assembled by yNap1 as a function of [Nap1] for unmodified nucleosomes (black square) and H3(T118ph) nucleosomes (gray squares).