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. 2014 Feb 21;42(8):4922–4933. doi: 10.1093/nar/gku150

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© The Author(s) 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Nucleosome duplexes and altosomes are twice the size of mononucleosomes. AFM images of purified (A) mp2-247 nucleosomes with unmodified HO (247 Unmod Canonical Nucs), (B) mp2-247 nucleosomes with H3(T118ph) HO (247 T118ph Canonical Nucs) and (C) mp2-247 nucleosome duplexes with H3(T118ph) HO (247 T118ph Nuc Duplexes/altosomes). Inset is 60 nm in width. Histograms of (D) surface area and (E) height of mp2-247 containing unmodified nucleosomes (black square, n = 173), H3(T118ph) canonical nucleosomes (blue circle, n = 140) and H3(T118ph) nucleosome duplexes/altosomes (red triangle, n = 175). AFM images of 601-17mer arrays reconstituted with (F) unmodified (11.2 ± 0.5 nucleosomes per molecule, n = 27) and (G) H3(T118ph) HO (7.1 ± 0.4 discrete complexes per molecule, n = 32). Inset is 400 nm in width.