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. 2014 Feb 11;42(8):4868–4881. doi: 10.1093/nar/gku135

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© The Author(s) 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — MS-based analyses of interacting interface of Brd4 with MLV IN. Representative sections of MALDI-ToF data for tryptic peptides of 6xHis–Brd4(1–720) are shown. Modification conditions are indicated to the left with the top profile: 6xHis–Brd4(1–720) alone; the middle profile: 6xHis–Brd4(1–720) plus 1 mM NHS-biotin; and the bottom profile: 6xHis–Brd4(1–720) preincubated with GST–MLV IN and then treated with 1 mM NHS-biotin. The peaks are indicated by their start and end amino acid numbers. The Lys residues modified by NHS-biotin are indicated in brackets. Also shown are peaks of peptides (445–453 and 646–665) whose intensities do not vary as they do not contain any modified lysine residues allowing them to serve as internal controls.