. 2014 Feb 11;42(8):4868–4881. doi: 10.1093/nar/gku135

Table 2.

Binding dissociation constants of various Brd4 fragments with full-length GST–MLV IN

	K_d (nM)
Brd4(1–720)	58.37 ± 9.57
nBrd4(1–461)	>2000^a
cBrd4(462–720)	77.30 ± 4.21
Brd4 B/BID(462–599)	>2000^a
Brd4 ET(600–678)	204.97 ± 12.45^*
Brd4 ET/SEED(600–720)	205.99 ± 11.58^*

Pulled-down 6xHis–Brd4 fragments bands were quantified using ImageJ software and data was fit with the Hill equation.

^aBinding was not detected at the indicated concentration.

*P < 0.0001 as measured by one-way ANOVA; multiple comparisons of the indicated Brd4 fragments to the Brd4(1–720) control used Dunnett simultaneous test, n = 3.