Skip to main content
. 2014 Feb 5;42(8):5109–5124. doi: 10.1093/nar/gku108

Figure 3.

Figure 3.

Purification of NmLeuRSs and optimization of aminoacylation conditions. (A) A total of 8% SDS-PAGE analysis of the purified NmLeuRSs from E. coli. Lanes: 1, 2 and 3 are molecular markers (Thermo Scientific, #26614), NmLeuRS1 and NmLeuRS2, respectively. (B) Relative aminoacylation activity of NmLeuRS1, EcLeuRS and PhLeuRS under different KCl or NaCl concentrations. The activities of EcLeuRS in 0.1 M KCl, PhLeuRS in 0 M KCl and NmLeuRS1 in 3.75 M KCl were defined as 100%. (C) Relative aminoacylation activities of NmLeuRS1 under different pH conditions in Tris–HCl (black circle), Bis-Tris-propane-HCl (black up-pointing triangle) and CHES-KOH (black square). The activity of NmLeuRS1 in Tris–HCl, pH 9.0, was defined as 100%.