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. 2014 Feb 5;42(8):5109–5124. doi: 10.1093/nar/gku108

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© The Author(s) 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — The editing properties for Nva by NmLeuRS1. (A) A representative image showing the formation of [³²P]AMP by 2 μM NmLeuRS1 in the absence (− tRNA) or presence (+ tRNA) of 25 μM NmtRNA^Leu. A 2-fold diluted [α-³²P]ATP for quantification (initial concentration, 4.5 nmol) is also shown. (B) A representative image showing the formation of [³²P]AMP by NmLeuRS1-D354A in the presence of NmtRNA^Leu. (C) Quantification of the formation of [³²P]AMP by NmLeuRS1without tRNA (black circle) and with tRNA (black square) and by NmLeuRS1-D354A with tRNA (black up-pointing triangle).