Table 1.
Thermodynamic parameters calculated for the E. coli TPP riboswitch with and without sequence variations in the k-junction
| k-junction | n | ΔH/kJ.mol−1 | ΔS/J.K-1.mol−1 | ΔG/kJ.mol−1 | Kd/μM |
|---|---|---|---|---|---|
| Natural | 1.07 ± 0.07 | –18.29 ± 0.18 | –30 ± 0.63 | –9.20 ± 0.06 | 0.23 ± 0.02 |
| A1bG | 0.56 ± 0.01 | –17.82 ± 0.45 | –33.1 ± 1.50 | –7.79 ± 0.06 | 2.44 ± 0.23 |
| ΔUC | 0.82 ± 0.01 | –8.72 ± 0.12 | 1.24 ± 0.47 | –9.09 ± 0.08 | 0.28 ± 0.04 |
| A1nU | 0.93 ± 0.04 | –11.81 ± 0.79 | –14.3 ± 2.63 | –7.48 ± 0.11 | 4.07 ± 0.73 |
| A2bC | 0.91 ± 0.02 | –21.85 ± 0.47 | –44.3 ± 1.57 | –8.42 ± 0.08 | 0.85 ± 0.11 |
| A2bU | 0.88 ± 0.01 | –21.18 ± 0.24 | –41.2 ± 0.81 | –8.69 ± 0.05 | 0.55 ± 0.04 |
Note that the A1nC variant is not included, because no binding was detectable with the data obtained; n is the stoichiometry of the interaction.