Figure 1.

K-H forms a complex with NHEJ factors. (A and B) To interrogate potential in vivo K-H interacting partners exponentially growing HeLa cells were collected and lysed for FPLC. Individual fractions were separated by SDS-PAGE and western blot techniques were used to probe for indicated proteins. (C) To validate K-H and NHEJ complex member interactions nuclear extracts from shScr cells were used to perform co-immunoprecipitation (co-IPs) experiments with and without EtBr (5 μM), to check for the contribution of DNA to these interactions. Co-IPs were separated by SDS-PAGE followed by western blot analysis for indicated NHEJ factor.